He aha ka QuickMice™?

Ua ʻōlelo ʻia ke kelepona he homozygous no kekahi gene ke loaʻa nā alleles like o ka gene ma nā chromosomes homologous ʻelua.

Loaʻa i nā hiʻohiʻona ʻiole i hoʻohana ʻia i nā ʻano noiʻi ma nā kahua noiʻi o ka maʻi AIDS, ka maʻi maʻi maʻi, nā maʻi ʻeha, a me nā maʻi koko.

ʻO Knock-in (KI) kahi ʻano hana e hoʻohana ai i ka homologous recombination o nā genes e hoʻololi i kahi ʻano hana exogenous i loko o kahi kaʻina homologous i loko o ke kelepona a me ka genome, a loaʻa ka hōʻike maikaʻi i loko o ke kelepona ma hope o ka hoʻohui hou ʻana.

ʻO Conditional Knock-out (CKO) kahi ʻenehana hoʻopaʻa kino kikoʻī kikoʻī i loaʻa e kahi ʻōnaehana hoʻohui kūloko.

Ma ke noi ʻanaTurboMice™ʻenehana, hiki iā mākou ke nānā pono i nā pūnaewele embryonic stem ma hope o ka hoʻoponopono ʻana o ka gene i nā lā 3-5, a laila kūkulu i kahi cell tetraploid, a loaʻa i nā ʻiole homozygous multi-locus gene-edited ʻiole i loko o 3-5 mau mahina ma hope o ka surrogacy e nā ʻiole makuahine, hiki ke mālama i 1 makahiki. no kā mākou mea kūʻai.

TurboMice™Hiki i ka ʻenehana ke hoʻoponopono pololei i nā ʻāpana lōʻihi ma luna o 20kb, no laila e hoʻomaʻamaʻa i ka hana wikiwiki ʻana o nā hiʻohiʻona paʻakikī e like me ke kanaka, conditional knockout (CKO), a me ka ʻāpana kīkē nui (KI).