ʻO ka ʻenehana hou aʻe e hoʻolalelale ana i ka hana ʻana i ke kumu hoʻohālike iole
ʻenehana TurboMice™
Hoʻomohala ʻia e MingCeler ma o kahi ʻano o ka optimizations o ka hoʻokō tetraploid.
Ma ka hui ʻanaʻenehana pololei Gene Editinga me ka ʻiole i hoʻonui ʻiaEmbryonic Stem Cell Preparation Technology, hiki iā mākou ke hoʻoponopono kokoke i nā locus gene target.
ʻenehana TurboMice™
ʻO kā mākou ʻenehana Tetraploid Complementation maikaʻi loa ua ʻae iā mākou e hoʻonui nui i ka nui o ka hānau ʻana o nā ʻiole mai 1% -5% a i 30% -60% me nā cell stem-edited stem cell, kokoke e hoʻohālikelike i ka pono o ka hoʻoili ʻana o ka embryo maʻamau.
ʻO MingCeler ka hui mua o ka honua i hoʻokō kūleʻa i ka hoʻololi ʻana o ka ʻenehana Tetraploid Complementation mai ke keʻena hana i ka noi ʻoihana.
ʻenehana kuʻuna
ʻO ka ʻenehana hoʻohālike holoholona kuʻuna, e like me ka microinjection pronuclear a me ka huli ʻana o ES, pili i ka hānau ʻana ma ka liʻiliʻi 2 a 3 mau hanauna e loaʻa ai nā ʻiole o kahi genotype homozygous, ka mea maʻamau i 6-8 mau mahina.
ʻO kēia kaʻina hana lōʻihi e pale nui i ka holomua a me ka maikaʻi o ka hoʻomohala lāʻau hou a me ka noiʻi maʻi.
Puʻupuʻu wikiwiki
1. a hiki i 20 mau ʻiole homozygous gene-edited i loko2-4 mahina.
2. ≈ 50 mau ʻiole homozygous i loko o kēia mau mea2 mahina.
3. 400+ ʻiole homozygous i loko8-12 mahina.
Hōʻike hihia ma 2020
Ua hoʻohana ʻia ka ʻenehana TurboMice™ e hana wikiwiki500 homozygouskanaka iole ACE2 i loko wale nō8 mahinano ka noiʻi ʻana i ka lāʻau lapaʻau COVID-19 a me ke kano.
Koho ʻoluʻolu
He palena koʻikoʻi ko nā ʻenehana kuʻuna i ke koho ʻana i ka ʻiole, ʻoiai hāʻawi ka ʻenehana TurboMice™ i ka maʻalahi me nā ʻano ʻano inbred a outbred e koho ai (me Balb /c, ICR, C57BL/6, etc.).
● ʻO nā ʻiole hanauna F0 i kūkulu ʻia me ka ʻenehana TurboMice™ ʻo ia nā ʻiole i manaʻo ʻia me ka hoʻomaka ʻana o ke kelepona hoʻokahi.
● No laila, ʻano like ke ʻano o nā ʻiole F0, e hōʻemi ana i nā hewa hoʻokolohua i hoʻokumu ʻia e nā ʻano like ʻole.
● No laila, ʻoi aku ka kūpaʻa a me ka hilinaʻi o ka ʻikepili hoʻokolohua no ka loiloi pono o ka lāʻau a me nā hoʻokolohua ʻē aʻe.
Mālama i ka Pono Genetic maikaʻi
In Situ Precision Gene Editing
● In situ ka hoʻoponopono gene kikoʻī me nā pae hōʻike gene pololei a me ka kiko kiko kiko kiko.
● Hoʻokumu ʻia nā ʻiole ACE2 kanaka i hoʻomohala ʻia ma o nā ʻenehana kuʻuna e ka hoʻolauna exogenous o ka mea hoʻolaha K18-ACE2, ʻaʻole hiki ke hoʻokō pono i ka hoʻoponopono pololei ʻana ma muli o ka hoʻokomo ʻole ʻana, ka hopena i ka nele o ke kiko kikoʻī o ka ʻōlelo ACE2 i ke kumu hoʻohālike kiʻi kanaka ACE2 i kūkulu ʻia. .
● Hōʻike ʻia nā ʻiole ACE2 i hana ʻia e MingCeler i nā ʻano kikoʻī i loko o nā kino like ʻole (kiʻi C a me D ma luna), a hoʻohālikelike pono i nā ʻano lapaʻau ma hope o ka maʻi SARS-CoV-2.
Unique EnhancerPlus Platform
●Hiki i ka MingCeler's proprietary EnhancerPlus platform ke kōkua i kā mākou mea kūʻai aku e hoʻomaikaʻi i nā pae hōʻike o nā genes kanaka.
● ʻO ka hopena o nā makahiki o ka hōʻiliʻili ʻikepili i ka epigenetics a me ka noiʻi hoʻoholo ʻana o ka cell fate, hiki i ka MingCeler's EnhancerPlus platform ke wānana pololei i nā kūlana genomic o Enhancers, e hana ana i ka hoʻolālā ʻana o nā kumu hoʻoponopono gene i kumu hōʻike.
● ʻO ka hopena, hiki ke hoʻohālikelike ʻia nā ʻano genes i ʻike ʻia me nā ʻano hōʻike a me nā pae o nā genes endogenous.
Hōʻike hihia: Humanization o X gene.
● Leveraging the EnhancerPlus platform, the plang was optimized and the expression level i ike i ka piʻi ʻana o ʻekolu kauoha o ka nui (e nānā ma luna o ke kiʻi), hiki i nā koi no ka hoʻomohala lāʻau e like me ka mea i hoʻoholo ʻia e ka mea kūʻai ma ka pae protein.
● Ma hope o ka hoʻopau ʻana i ka hana nui o ka honua i nā ʻiole kanaka ACE2 i ka makahiki 2020, ua hoʻomaikaʻi ʻo MingCeler i ke kumu hoʻohālike ʻiole kanaka ACE2 ma o ʻehā iterations ma o EnhancerPlus optimization, me ke kiʻekiʻe o ka hōʻike kanaka ACE2 a hiki i kahi kokoke i ka hōʻike endogenous o ka ʻiole ACE2.
Nā pae hōʻike o ke kanaka ACE2 i nā ʻāpana like ʻole o nā kiʻi kiʻi kanaka
Hoʻoponopono Gene Multi-Locus
● ʻO ka ʻenehana TurboMice™ ka koho maikaʻi loa ma ka mākeke no ka hana ʻana i nā hiʻohiʻona ʻiole i hoʻoponopono ʻia i nā ʻano multi-locus combinatorial gene-edited, e hāʻawi ana i nā pono o ka pololei kiʻekiʻe i ka hoʻokomo ʻana i ka wahi, ka hoʻokomo ʻana i nā ʻāpana lōʻihi, a me nā loci hoʻololi genetically, me ka pilikia ʻole o ka hoʻokaʻawale allelic.
● ʻAe ka ʻenehana TurboMice™ i ka hoʻoponopono like ʻana a hiki i 3 mau genes e hana i nā hiʻohiʻona ʻiole homozygous multi-locus gene-edited ʻiole pololei mai nā pūnaewele embryonic stem cell i hoʻoponopono ʻia, me ka ʻole o nā kaʻina hana hoʻolāʻau / nānā ʻana, e hāʻawi ana i nā ʻano like ʻole. o nā kumu hoʻohālike paʻakikī waiwai nui e pono ai no ka noiʻi lāʻau hou.
● Ua hoʻomohala maikaʻi ʻo MingCeler i nā ʻano ʻiole multi-loci gene-edited i ka manawa pōkole, ma muli o nā ʻiole kanaka ACE2.
Hoʻoponopono Gene Fragment lōʻihi
Hiki i ka ʻenehana TurboMice™ ke hoʻoponopono pololei i nā ʻāpana lōʻihi o ka 20kb, e hoʻomaʻamaʻa i ka hana wikiwiki ʻana o nā hiʻohiʻona paʻakikī e like me ke kanaka, conditional knock-out (CKO), a me ka ʻāpana kīkē nui (KI).
Hoolaha
[1] Wang G, Yang ML, Duan ZL, Liu FL, Jin L, Long CB, Zhang M, Tang XP, Xu L, Li YC, Kamau PM, Yang L, Liu HQ, Xu JW, Chen JK, Zheng YT , Peng XZ, Lai R. Dalbavancin i hoʻopaʻa i ka ACE2 e ālai i kāna pilina me SARS-CoV-2 spike protein a he mea kūpono ia i ka pale ʻana i ka maʻi SARS-CoV-2 i nā ʻano holoholona.Kelepona Res.2021 Ian;31(1):17-24.doi: 10.1038/s41422-020-00450-0.( IF: 20.507 )
[2] Liu FL, Wu K, Sun J, Duan Z, Quan X, Kuang J, Chu S, Pang W, Gao H, Xu L, Li YC, Zhang HL, Wang XH, Luo RH, Feng XL, Schöler HR , Chen X, Pei D, Wu G, Zheng YT, Chen J. ʻO ka hanauna wikiwiki o ACE2 i hoʻohālikelike ʻia i ka ʻiole inbred kanaka no COVID-19 me ka hoʻokō tetraploid.Natl Sci Rev. 2020 Nov 24;8(2): nwaa285.doi: 10.1093/nsr/nwaa285.( IF: 16.693 )
Kawai lawelawe
ʻO nā mea a pau āu e hana ai, ʻo ka hāʻawi ʻana iā mākou i kāu koi hoʻololi gene no kāu kumu hoʻohālike ʻiole.Ma muli o kāu mau kikoʻī, e hoʻokomo mākou i kahi hoʻolālā mua i hoʻonohonoho ʻia, a ma hope o ke kūkākūkā hou ʻana a me ka ʻaelike like, e kau inoa mākou i kahi ʻaelike lawelawe ʻenehana.Ma hope o ka hoʻomaka ʻana o ka papahana e hāʻawi mākou i nā mea hou i ka manawa kūpono no ka holomua.